Inoki Reizo, Hayashi Takeshi, Kudo Teruo, Matsumoto Ken
Department of Pharmacology, Osaka University Dental School, Osaka 530 Japan.
Pain. 1978 Jun;5(1):53-63. doi: 10.1016/0304-3959(78)90024-6.
The effects of aspirin and morphine on the release of bradykinin-like substance into the substaneous perfusate following various noxious stimuli were investigated in rat paw preparation. Subcutaneous perfusion was performed with saline or with saline containing o-phenanthroline or soy bean trypsin inhibitor at a rate of 3--4 drops/min. Ten drops per fraction were assayed for bradykinin activity. Pressure on the instep, application of heat to the foot and stimulation of the sciatic nerve were employed as noxious stimuli. Aspirin (200 mg/kg i.p.) inhibited the release of bradykinin due to heat, pressure and sciatic nerve stimulation, while morphine (5 mg/kg i.m.) inhibited only the release of bradykinin due to sciatic nerve stimulation. Results suggest that analgesics, such as aspirin and morphine, have a depressant action on pain by inhibiting the release of bradykinin locally. They also suggest that aspirin inhibits the release of bradykinin regardless of the type of noxious stimuli, while morphine inhibits the release of bradykinin which is mediated by neural mechanisms.
在大鼠爪部制备模型中，研究了阿司匹林和吗啡对各种伤害性刺激后缓激肽样物质释放到皮下灌流液中的影响。用生理盐水或含邻菲罗啉或大豆胰蛋白酶抑制剂的生理盐水以3 - 4滴/分钟的速度进行皮下灌流。每部分取十滴检测缓激肽活性。采用足背加压、足部加热和坐骨神经刺激作为伤害性刺激。阿司匹林（200毫克/千克腹腔注射）抑制因热、压力和坐骨神经刺激引起的缓激肽释放，而吗啡（5毫克/千克肌肉注射）仅抑制因坐骨神经刺激引起的缓激肽释放。结果表明，阿司匹林和吗啡等镇痛药通过抑制局部缓激肽释放对疼痛具有抑制作用。结果还表明，阿司匹林无论伤害性刺激类型如何均能抑制缓激肽释放，而吗啡抑制由神经机制介导的缓激肽释放。
