Ingram G A, Molyneux D H
Dev Comp Immunol. 1984 Spring;8(2):339-49. doi: 10.1016/0145-305x(84)90040-5.
The response of the spiny-tailed agamid lizard, Agama caudospinosum, to administration of Leishmania agamae promastigotes was investigated. Lizards given a single injection of promastigotes showed no signs of clinical infection. Neither promastigotes nor amastigotes were found in blood and tissue impression smears, nor in blood and selected body organ cultures. However, parasite antigens were demonstrated by an immunoenzyme method only in the liver, small intestine, stomach, spleen and kidney. Non-precipitating serum antibodies with gamma-electrophoretic mobility were detected by enzyme-lined immunosorbent assay 1 week post-injection and a maximum titre was reached after 6 weeks. The mean immune serum protein concentration increased significantly (P less than 0.005) about two-fold over the controls after injection. Decreases occurred in the beta-globulin region of anti-L. agamae sera (P less than 0.01) whilst the gamma-globulin fraction was increased (P less than 0.005) following electrophoresis on cellulose acetate membranes. C-reactive protein was not detected in any of the sera. These data show that although A. caudospinosum failed to become infected by L. agamae promastigotes, which had been isolated from agamids, it did exhibit antigen distribution and a humoral response similar to other reptiles.
对棘尾鬣蜥（Agama caudospinosum）接种来自鬣蜥的利什曼原虫前鞭毛体后的反应进行了研究。单次注射前鞭毛体的蜥蜴未表现出临床感染迹象。在血液和组织印片中以及血液和选定的身体器官培养物中均未发现前鞭毛体和无鞭毛体。然而，仅在肝脏、小肠、胃、脾脏和肾脏中通过免疫酶法检测到了寄生虫抗原。注射后1周通过酶联免疫吸附测定法检测到具有γ电泳迁移率的非沉淀血清抗体，6周后达到最高滴度。注射后，平均免疫血清蛋白浓度比对照组显著增加（P<0.005），约为对照组的两倍。在醋酸纤维素膜上进行电泳后，抗利什曼原虫血清的β球蛋白区域减少（P<0.01），而γ球蛋白部分增加（P<0.005）。在任何血清中均未检测到C反应蛋白。这些数据表明，尽管棘尾鬣蜥未被从鬣蜥分离出的利什曼原虫前鞭毛体感染，但它确实表现出与其他爬行动物相似的抗原分布和体液反应。
