刺尾鬣蜥（Agama caudospinosum）对注射利什曼原虫鞭毛体（Leishmania agamae promastigotes）的体液免疫反应。

The humoral immune response of the spiny-tailed agamid lizard (Agama caudospinosum) to injection with Leishmania agamae promastigotes.

作者信息

Ingram G A, Molyneux D H

出版信息

Vet Immunol Immunopathol. 1983 May;4(4):479-91. doi: 10.1016/0165-2427(83)90008-9.

Abstract

Spiny-tailed agamid lizards (Agama caudospinosum) were given a single intraperitoneal injection of Leishmania agamae promastigotes. Direct agglutinins (DA), indirect haemagglutinins (IHA) and complement-fixing antibodies (CFA) produced against the parasites were non-precipitating, relatively thermostable and dithiothreitol sensitive. Antibodies were also detected by the immobilisation test (IMM) and by enzyme-linked immunosorbent assay (ELISA). The most sensitive method was the ELISA one. Antibodies were detected 7 days post-injection and maximum IMM (2(-6)), DA (2(-8)), CFA (2(-10)), IHA (2(-12)) and ELISA (2(-16)) titres were obtained from 35 to 49 days. In all cases, the levels of antibody following antigenic stimulation were significantly different from the controls (P less than 0.001). Serum lysozyme levels increased three-fold (P less than 0.001) with the highest value of 46 micrograms/ml occurring after 42 days.

摘要

给刺尾鬣蜥（Agama caudospinosum）腹腔内单次注射利什曼原虫前鞭毛体。针对这些寄生虫产生的直接凝集素（DA）、间接血凝素（IHA）和补体结合抗体（CFA）不沉淀、相对耐热且对二硫苏糖醇敏感。抗体也通过固定试验（IMM）和酶联免疫吸附测定（ELISA）检测到。最敏感的方法是ELISA法。注射后7天检测到抗体，在35至49天获得最大IMM（2⁻⁶）、DA（2⁻⁸）、CFA（2⁻¹⁰）、IHA（2⁻¹²）和ELISA（2⁻¹⁶）滴度。在所有情况下，抗原刺激后抗体水平与对照组有显著差异（P小于0.001）。血清溶菌酶水平增加了三倍（P小于0.001），在42天后达到最高值46微克/毫升。