Ingram G A, Molyneux D H
Parasite Immunol. 1983 Jan;5(1):95-108. doi: 10.1111/j.1365-3024.1983.tb00727.x.
European green lizards, Lacerta viridis, produced relatively thermostable, dithiothreitol-sensitive, non-precipitating, agglutinins and complement-fixing antibodies (CFA) to Leishmania agamae administered subcutaneously (SC), intraperitoneally (IP) or orally (OR). Antibodies were also detected by the immobilization test (IMM) and by enzyme-linked immunosorbent assay (ELISA). The most sensitive method for the detection of stimulated immunoglobulins was ELISA. Antibodies were detected as early as 3 days post-infection with ELISA and between 5 and 7 for CFA, direct agglutination (DA) and indirect haemagglutination (IHA). In the case of IMM, the times of first detection varied from 14 to 28 days. Maximum CFA (2(-8)), DA (2(-8)), IHA (2(-11)) and ELISA (2(-16)) titres were reached from 42 to 49 days with significantly higher values occurring in the OR and IP groups. With IMM, maxima occurred after 5 or 6 weeks. Following exposure, two- to five-fold significant increases in serum lysozyme levels were demonstrated but the concentrations in sera following SC, IP or OR routes of antigen administration were not significantly different when the groups were compared with each other. The highest lysozyme values (approximately 12.3 - 12.5 micrograms ml(-1)) were found in the SC and OR groups when compared to the IP (7.40 micrograms ml(-1)).
欧洲绿蜥（Lacerta viridis）对经皮下（SC）、腹腔内（IP）或口服（OR）接种的阿加马利什曼原虫产生了相对耐热、对二硫苏糖醇敏感、不沉淀的凝集素和补体结合抗体（CFA）。还通过固定试验（IMM）和酶联免疫吸附测定（ELISA）检测到了抗体。检测刺激产生的免疫球蛋白最灵敏的方法是ELISA。感染后3天用ELISA即可检测到抗体，CFA、直接凝集（DA）和间接血凝（IHA）则在5至7天检测到。对于IMM，首次检测时间在14至28天之间。42至49天时达到最大CFA（2⁻⁸）、DA（2⁻⁸）、IHA（2⁻¹¹）和ELISA（2⁻¹⁶）滴度，OR组和IP组的值显著更高。对于IMM，最大值出现在5或6周后。接触抗原后，血清溶菌酶水平显著升高2至5倍，但比较各给药组（SC、IP或OR）血清中的溶菌酶浓度时，差异不显著。与IP组（7.40微克/毫升）相比，SC组和OR组的溶菌酶值最高（约12.3 - 12.5微克/毫升）。
