Balduini C L, Sinigaglia F, Salvini P, Balduini C
Haemostasis. 1984;14(2):158-63. doi: 10.1159/000215051.
Human washed platelets were eluted from columns of Sepharose 4B linked to different preparations of collagen in order to evaluate cell adhesion. Collagen preparations characterized by low and high affinity toward platelets were identified. In our experiments, fibronectin purified from human plasma modified platelet adhesiveness, though not dramatically. When washed platelets, resuspended in a buffer containing fibronectin, were filtered on a low-affinity collagen-Sepharose, a significant increase in their adhesion occurred. A similar modification could be observed when platelets were allowed to adhere to the same collagen-Sepharose preconditioned with fibronectin. The effect of fibronectin was otherwise negligible when the high-affinity collagen was used for the experiments.
从与不同胶原蛋白制剂相连的琼脂糖4B柱上洗脱人洗涤血小板，以评估细胞黏附。鉴定出了对血小板具有低亲和力和高亲和力的胶原蛋白制剂。在我们的实验中，从人血浆中纯化的纤连蛋白改变了血小板的黏附性，尽管变化不显著。当将悬浮于含纤连蛋白缓冲液中的洗涤血小板过滤通过低亲和力胶原-琼脂糖时，其黏附显著增加。当血小板黏附于用纤连蛋白预处理的相同胶原-琼脂糖时，也可观察到类似的改变。然而，当使用高亲和力胶原进行实验时，纤连蛋白的作用可忽略不计。
