Fauvel F, Legrand Y, Lebret M, Caen J P
Pathol Biol (Paris). 1976 Dec;24 Suppl:70-3.
14C serotonine labelled platetel rich plasma (PRP) incubated with fibrillar collagen is either gel filtered through a Sepharose 2B column (Sepharose test) or centrifugated on a 23% Ficoll layer (Ficoll test). In the Sepharose test, the adhesion is quantified by the determination of the per cent yield of free platelets eluted from the column and the release by a quantitative evaluation of the 14C serotonin. In the Ficoll test, the released seroton is measured from the distribution of radioactivity in the different layers (supernatant plasma, platelets adherent to collagen, Ficoll, platelet pellet) of the tube after centrifugation. Both methods are rapid and quite reproducible.
将用14C血清素标记的富含血小板血浆（PRP）与纤维状胶原一起孵育，然后通过琼脂糖2B柱进行凝胶过滤（琼脂糖试验），或者在23%的聚蔗糖层上进行离心（聚蔗糖试验）。在琼脂糖试验中，通过测定从柱中洗脱的游离血小板的百分回收率来定量粘附，并通过对14C血清素的定量评估来测定释放量。在聚蔗糖试验中，通过离心后管中不同层（上清血浆、粘附于胶原的血小板、聚蔗糖、血小板沉淀）中放射性的分布来测量释放的血清素。这两种方法都快速且相当可重复。
