Ferruchhi L, Sumner A T
Histochem J. 1984 May;16(5):521-8. doi: 10.1007/BF01041352.
Experiments have been carried out to investigate the DNA base specificity of light-induced banding (LIB) produced by photo-oxidation of chromosomes followed by Acridine Orange staining to detect denatured DNA. Nuclei of different base composition, human and onion, and fluorochromes of different base specificities and modes of binding to DNA were used. Our results indicate that specific destruction of guanine residues is the main effect of photo-oxidation under the conditions used, and that LIB is a base-specific phenomenon. In addition, photo-oxidation may also cause DNA-protein cross-linking which affects the binding of some dyes, while prolonged photo-oxidation appears to cause more general damage to DNA.
已开展实验，研究染色体光氧化后通过吖啶橙染色检测变性DNA所产生的光诱导带（LIB）的DNA碱基特异性。使用了不同碱基组成的细胞核（人类和洋葱）以及与DNA具有不同碱基特异性和结合模式的荧光染料。我们的结果表明，在所使用的条件下，鸟嘌呤残基的特异性破坏是光氧化的主要效应，并且LIB是一种碱基特异性现象。此外，光氧化还可能导致DNA - 蛋白质交联，从而影响某些染料的结合，而长时间的光氧化似乎会对DNA造成更普遍的损伤。
