Time-temperature relationship for hyperthermia induced stoppage of the microcirculation in tumors.

The time-temperature dependence for microcirculation stoppage was investigated for the Rhabdomyosarcoma BA1112 growing in observation chambers ("sandwich chambers"). The tumor microcirculation could be observed continuously during the treatment, and the condition of the microcirculation was recorded every 15 minutes as "flowing" or "stoppage". By using large numbers of tumors, the 50% stoppage time (ST50) could be derived for the four temperatures investigated: 42 degrees, 42.5 degrees, 43 degrees and 43.5 degrees C. The respective ST50 values were 226, 152, 101 and 70 minutes. The results can be expressed as a log-linear relationship with a slope value of 0.4551 +/- 0.03 (SD) per degree centigrade. This value probably does not differ significantly from the "t 1/2 for every degree C" rule that has been found for the thermal response of many biological systems.