Bhide S V, Gothoskar S V, Shivapurkar N M
J Cancer Res Clin Oncol. 1984;107(3):169-71. doi: 10.1007/BF01032602.
Arecoline, a major alkaloid present in betel nut, was administered daily by gavage feeding to Swiss male and female mice at a dose of 1 mg/day/mouse five times a week, either alone or in combination with KNO3 or KNO3 + lime. Swiss mice of both sexes kept on a vitamin B complex-deficient diet were tested in a similar manner and compared with those receiving a normal diet. In the mice receiving a normal diet it was observed that arecoline induced tumors in 40% of males but failed to produce tumors in any of the females. Arecoline tumorigenicity in females was evident only when they received a vitamin B-deficient diet. Arecoline tumorigenicity was not evident in males when they were treated simultaneously with KNO3 + lime and kept on a normal diet. However, the same treatment administered to male mice kept on a vitamin B complex-deficient diet induced tumors in 39%.
槟榔碱是槟榔中的一种主要生物碱，每周5天，以1毫克/天/只的剂量通过灌胃方式每日给予瑞士雄性和雌性小鼠，单独给药或与硝酸钾或硝酸钾+石灰联合给药。以类似方式对食用复合维生素B缺乏饮食的瑞士雌雄小鼠进行测试，并与食用正常饮食的小鼠进行比较。在食用正常饮食的小鼠中，观察到槟榔碱在40%的雄性小鼠中诱发肿瘤，但在任何雌性小鼠中均未诱发肿瘤。仅当雌性小鼠食用维生素B缺乏饮食时，槟榔碱的致瘤性才明显。当雄性小鼠同时接受硝酸钾+石灰处理并保持正常饮食时，槟榔碱的致瘤性不明显。然而，对食用复合维生素B缺乏饮食的雄性小鼠进行相同处理时，39%的小鼠诱发了肿瘤。
