Pai S R, Shirke A J, Gothoskar S V
Carcinogenesis. 1981;2(3):175-7. doi: 10.1093/carcin/2.3.175.
The observations on the effect of 3 agents--1,4-dinitrosopiperazine, betel nut and saccharin fed to C17 mice in combination is presented in this report. A total of 119 inbred mice of both sexes were put on long-term feeding trials. Group I consisted of 34 mice given a standard diet; group II of 32 mice fed an experimental diet containing saccharin coated betel nut powder at 10% concentration; group III of 29 mice given 0.2 ml aqueous solution of 0.1% 1,4-dinitrosopiperazine by intubation daily and group IV of 24 mice fed a combination of the experimental diet together with intubation of 1,4-dinitrosopiperazine. Feeding was continued for 40 weeks at which time all mice were given a standard diet and water ad libitum, and then observed for their full life-span. The commonest neoplasm found was squamous cell carcinoma of the forestomach in groups III and IV. Male mice were more susceptible to the treatment than female mice. In female mice reticular cell neoplasm--Type A of the uterus was the commonest tumour and was more common in group III than in group IV. The diet of saccharin coated betel nut failed to potentiate the carcinogenicity of 1,4-dinitrosopiperazine.
本报告介绍了对1,4 - 二亚硝基哌嗪、槟榔和糖精三种试剂联合投喂C17小鼠的效果观察。总共119只雌雄同体的近交系小鼠进行了长期喂养试验。第一组由34只给予标准饮食的小鼠组成；第二组由32只喂食含10%浓度糖精包衣槟榔粉实验饮食的小鼠组成；第三组由29只每天经插管给予0.1% 1,4 - 二亚硝基哌嗪水溶液0.2毫升的小鼠组成；第四组由24只喂食实验饮食并经插管给予1,4 - 二亚硝基哌嗪的小鼠组成。喂养持续40周，此时所有小鼠自由采食标准饮食和饮水，然后观察其整个寿命期。在第三组和第四组中发现最常见的肿瘤是前胃鳞状细胞癌。雄性小鼠比雌性小鼠对这种处理更敏感。在雌性小鼠中，子宫A型网状细胞瘤是最常见的肿瘤，且在第三组中比在第四组中更常见。糖精包衣槟榔饮食未能增强1,4 - 二亚硝基哌嗪的致癌性。
