Lachica R V, Zink D L
J Clin Microbiol. 1984 May;19(5):660-3. doi: 10.1128/jcm.19.5.660-663.1984.
A quick and simple method was developed to distinguish hydrophobic from hydrophilic cells. The latex particle agglutination test is based on the hydrophobic interactions between cells and latex particles which result in the agglutination of the suspension mixture. There was a direct correlation between the expression of plasmid-associated cell surface properties and latex particle agglutination by Yersinia enterocolitica. Multivalent cation-induced agglutination of suspensions of washed cells of virulent Y. enterocolitica and latex particles is indicative of their amphipathic character. Electrostatic interaction may also play a role in the latex particle agglutination reaction.
开发了一种快速简便的方法来区分疏水性和亲水性细胞。乳胶颗粒凝集试验基于细胞与乳胶颗粒之间的疏水相互作用，这种相互作用导致悬浮混合物发生凝集。小肠结肠炎耶尔森菌的质粒相关细胞表面特性表达与乳胶颗粒凝集之间存在直接相关性。多价阳离子诱导的强毒小肠结肠炎耶尔森菌洗涤细胞悬液和乳胶颗粒的凝集表明它们具有两亲性。静电相互作用也可能在乳胶颗粒凝集反应中起作用。
