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小肠结肠炎耶尔森菌的质粒相关细胞表面电荷与疏水性

Plasmid-associated cell surface charge and hydrophobicity of Yersinia enterocolitica.

作者信息

Lachica R V, Zink D L

出版信息

Infect Immun. 1984 May;44(2):540-3. doi: 10.1128/iai.44.2.540-543.1984.

DOI:10.1128/iai.44.2.540-543.1984
PMID:6715050
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC263563/
Abstract

Virulent strains of Yersinia enterocolitica and their plasmidless, avirulent derivatives were examined for their cell surface properties. Increased surface charge and hydrophobicity of Y. enterocolitica were found to be associated with the possession of a 40- to 48-megadalton plasmid. These surface properties were expressed, as were other plasmid-associated properties, at 37 but not at 22 degrees C. The concentration of calcium in the growth medium had a moderate effect on the expression of the cell surface properties. These cell surface properties were greatly reduced among plasmid-bearing cells grown on tryptic soy agarose regardless of growth temperatures. These properties were also associated with the ability of Y. enterocolitica to colonize the gastrointestinal tract of mice.

摘要

对小肠结肠炎耶尔森氏菌的强毒株及其无质粒无毒衍生物的细胞表面特性进行了研究。发现小肠结肠炎耶尔森氏菌表面电荷和疏水性的增加与拥有一个40至48兆道尔顿的质粒有关。这些表面特性与其他质粒相关特性一样,在37℃时表达,但在22℃时不表达。生长培养基中钙的浓度对细胞表面特性的表达有中等程度的影响。无论生长温度如何,在胰蛋白胨大豆琼脂糖上生长的携带质粒的细胞中,这些细胞表面特性都大大降低。这些特性还与小肠结肠炎耶尔森氏菌在小鼠胃肠道定殖的能力有关。

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