Khan M F, Ali S, Singh S V, Rahman Q, Viswanathan P N
J Appl Toxicol. 1984 Apr;4(2):87-91. doi: 10.1002/jat.2550040206.
The biochemical changes in rat lungs due to intratracheal instillation of 50 mg of slate dust have been studied up to 150 days of dust exposure. The remarkable feature of lung changes was the turnover of collagen in experimental animals after 90 days, reaching substantially higher values at 150 days. A concurrent increase in hexosamine and sialic acid contents was also observed. The phospholipid content in the whole lung tissue, as well as in the mitochondria, was generally higher in the dust-treated rats, particularly at the later stages. The mitochondrial cytochrome c oxidase and glutamate dehydrogenase activities increased, whereas monoamine oxidase was marginally affected. Mitochondria from experimental animals appeared to be in a swollen state, particularly at 120 days of exposure. The above results suggest that slate dust exerts its toxic effects by causing alterations in the tissue make-up as well as in the mitochondrial functioning of the lung.
对大鼠气管内注入50毫克板岩粉尘后肺部的生化变化进行了研究，观察期长达暴露于粉尘后的150天。肺部变化的显著特征是，实验动物在90天后胶原蛋白出现更新，在150天时达到更高水平。同时还观察到氨基己糖和唾液酸含量增加。经粉尘处理的大鼠全肺组织以及线粒体中的磷脂含量普遍较高，尤其是在后期。线粒体细胞色素c氧化酶和谷氨酸脱氢酶活性增加，而单胺氧化酶仅受到轻微影响。实验动物的线粒体似乎处于肿胀状态，尤其是在暴露120天时。上述结果表明，板岩粉尘通过引起肺部组织构成以及线粒体功能的改变来发挥其毒性作用。
