Antonowicz J, Andrzejak R, Smolik R
Chair and Clinic of Internal and Occupational Diseases, Medical Academy, Wrozław, Poland.
Int Arch Occup Environ Health. 1990;62(3):195-8. doi: 10.1007/BF00379431.
Exposure of foundry workers to mixtures of different heavy metals is a very important toxicological problem. In this paper the estimation of the effects of lead, zinc, and copper on erythrocyte metabolism is presented. Concentrations of copper and zinc at work posts of the group examined did not exceed TLV, while lead concentration was 1.5 to 4 times higher than TLV. Erythrocyte metabolism was measured through activities of such glycolytic pathway enzymes as PFK, PGI, PK, aldolase and G6-PD from the hexose monophosphate pathway. Additionally the free erythrocyte protoporphyrin (FEP) level, D-ALA activity, serum GSH level, 2,3 DPG level in erythrocytes and lactic acid production during a 2-h incubation of red blood cells (RBC) was estimated. The blood-lead level, FEP level, copper concentration in erythrocytes in exposed group were significantly higher than in control group while the zinc level in erythrocytes was significantly lower. Measuring erythrocyte metabolism we showed that the activity of PGI, PFK, aldolase, lactic production and 2,3 DPG levels was significantly higher in the exposed group, probably as a result of anaerobic glycolysis activation.
铸造工人接触不同重金属混合物是一个非常重要的毒理学问题。本文介绍了铅、锌和铜对红细胞代谢影响的评估。在所检查组的工作岗位上，铜和锌的浓度未超过阈限值（TLV），而铅浓度比阈限值高1.5至4倍。通过糖酵解途径的酶如磷酸果糖激酶（PFK）、磷酸葡萄糖异构酶（PGI）、丙酮酸激酶（PK）、醛缩酶以及来自磷酸己糖途径的葡萄糖-6-磷酸脱氢酶（G6-PD）的活性来测定红细胞代谢。此外，还估计了游离红细胞原卟啉（FEP）水平、δ-氨基乙酰丙酸（D-ALA）活性、血清谷胱甘肽（GSH）水平、红细胞中2,3-二磷酸甘油酸（2,3-DPG）水平以及红细胞（RBC）在2小时孵育期间的乳酸生成。暴露组的血铅水平、FEP水平、红细胞中的铜浓度显著高于对照组，而红细胞中的锌水平显著较低。通过测量红细胞代谢，我们发现暴露组中PGI、PFK、醛缩酶的活性、乳酸生成和2,3-DPG水平显著较高，这可能是无氧糖酵解激活的结果。
