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[使用光度法测定血清脂肪酶活性的临床研究]

[Clinical studies using a photometric method for determining serum lipase activity].

作者信息

Bornschein W

出版信息

Leber Magen Darm. 1984 May;14(3):125-8.

PMID:6738289
Abstract

Results of measurement of serum lipase activity as determined by a UV-test described by Myrick and a titrimetric assay as described by Rick were compared as far as sensitivity and specificity in diagnosis of pancreatitis is concerned. Sensitivity of the UV assay is low (45% falsely normal values), and specificity as well (falsely pathological values in 55.5% of patients with liver disease and 9% of normal persons). In the UV assay lipoproteinlipases are measured as well as hepatic esterases: in 17 samples from persons having received heparin lipase activity was increased 3-8 fold. In a total of 99 samples no correlation was found between the results of the 2 assays. As a consequence it can be stated that the UV-test as described by Myrick has no relevance in diagnosis of pancreatitis; the commercially available assay kit is withdrawn in the meantime.

摘要

就胰腺炎诊断的敏感性和特异性而言,对通过Myrick描述的紫外检测法测定的血清脂肪酶活性结果与Rick描述的滴定法测定结果进行了比较。紫外检测法的敏感性较低(45%的结果为假正常),特异性也较低(55.5%的肝病患者和9%的正常人结果为假病理)。在紫外检测法中,脂蛋白脂肪酶和肝酯酶都被检测:在17份来自接受肝素治疗者的样本中,脂肪酶活性增加了3至8倍。在总共99份样本中,两种检测结果之间未发现相关性。因此可以说,Myrick描述的紫外检测法与胰腺炎诊断无关;同时,该商业可用的检测试剂盒已被撤回。

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[Clinical studies using a photometric method for determining serum lipase activity].[使用光度法测定血清脂肪酶活性的临床研究]
Leber Magen Darm. 1984 May;14(3):125-8.
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[Comparison between a titrimetric and a photometric method for determination of serum lipase activity (author's transl)].
Med Klin. 1976 Feb 13;71(7):288-91.
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[Determination of lipase with 8-phenyloctanoic acid vinyl ester as the substrate].以8-苯基辛酸乙烯酯为底物测定脂肪酶
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In vivo interference of heparin bolus injection with photometric continuous lipase determination.肝素大剂量注射对连续光度法测定脂肪酶的体内干扰作用。
Eur J Clin Chem Clin Biochem. 1996 Apr;34(4):363-4.
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[Measurement of fecal chymotrypsin. Clinical and methodological aspects (author's transl)].[粪便糜蛋白酶的测定。临床与方法学方面(作者译)]
Leber Magen Darm. 1977 Oct;7(5):334-40.
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