Rick W, Hockeborn M
J Clin Chem Clin Biochem. 1982 Sep;20(9):627-32.
The UV-test described by Myrick ((1976) Ph. D. Thesis, Birmingham, AL, USA) for the determination of the catalytic activity of lipase in serum, using 8-phenyloctanoic acid vinyl ester as substrate, was investigated in detail. When tested with sera the assay gave unsatisfactory linearity. For some serum samples, evaluation was impossible, owing to the complete lack of a linear response. The substrate is attacked not only by purified pancreatic lipase, but also by liver esterase, cholinesterase in plasma, and lipoproteinlipase. Attempts to inhibit the interfering esterases with phenylmethylsulphonylfluoride were unsuccessful. Analyses of 102 sera showed no correlation with the results from the titrimetric assay (r = 0.158). Thus the method of Myrick does not provide clinically useful results.
详细研究了迈里克（（1976年）美国阿拉巴马州伯明翰博士论文）描述的以8-苯基辛酸乙烯酯为底物测定血清中脂肪酶催化活性的紫外线检测法。用血清进行检测时，该测定法的线性度不理想。对于一些血清样本，由于完全缺乏线性响应，无法进行评估。该底物不仅会受到纯化的胰脂肪酶的攻击，还会受到肝脏酯酶、血浆中的胆碱酯酶和脂蛋白脂肪酶的攻击。用苯甲基磺酰氟抑制干扰性酯酶的尝试未成功。对102份血清的分析表明，其结果与滴定法测定结果无相关性（r = 0.158）。因此，迈里克的方法无法提供临床有用的结果。
