Shank M L, Singh S P, Blivaiss B B, Kabir M A, Williams K, Premachandra B N
Metabolism. 1984 Jul;33(7):667-71. doi: 10.1016/0026-0495(84)90068-4.
Adult male rats were placed on a 3 week regimen of ethanol (as 20% of total calories) in a nutritionally adequate diet, and controls were matched equicalorically without ethanol. Serum measurements of T4, T3, FT4, rT3, and TSH were performed in both the fed and the fasted state (18 hours). In the fed state, serum hormone measurements did not differ between control and ethanol-treated rats. Overnight fasting had a significant effect in decreasing serum T3 level in both experimental and control rats and in decreasing serum T4 level in ethanol-treated animals; FT4 and rT3 levels were not affected. Fasting also decreased in vitro hepatic T4 to T3 production to an equivalent degree in control and ethanol-treated rats, but did not alter hepatic T4 to rT3 production rates in control animals. In the fed state, hepatic rT3 neogenesis in animals given ethanol declined relative to the levels observed in control fed rats; fasting restored the depressed rT3 neogenesis to the levels noted in the fed state. Because decreased rT3 production in ethanol-treated rats in the fed state could not be explained on the basis of a change in 5'-deiodinase activity, it is suggested that ethanol administered with a nutritionally adequate diet may inhibit hepatic rT3 generation by inhibiting T4(5)-deiodinase.
成年雄性大鼠被置于一种营养充足的饮食方案中，其中乙醇占总热量的20%，持续3周，对照组则给予等热量但不含乙醇的饮食。在喂食和禁食状态（18小时）下均对血清中的T4、T3、FT4、反T3（rT3）和促甲状腺激素（TSH）进行了测量。在喂食状态下，对照组和经乙醇处理的大鼠血清激素测量结果没有差异。过夜禁食对降低实验大鼠和对照大鼠的血清T3水平以及经乙醇处理动物的血清T4水平有显著影响；FT4和rT3水平不受影响。禁食还使对照组和经乙醇处理的大鼠肝脏中T4向T3的体外转化率同等程度降低，但未改变对照动物肝脏中T4向rT3的转化率。在喂食状态下，给予乙醇的动物肝脏中rT3的新生相对于喂食的对照大鼠所观察到的水平有所下降；禁食使降低的rT3新生恢复到喂食状态下所记录的水平。由于在喂食状态下经乙醇处理的大鼠中rT3生成减少不能基于5'-脱碘酶活性的变化来解释，因此提示在营养充足的饮食中给予乙醇可能通过抑制T4(5)-脱碘酶来抑制肝脏中rT3的生成。
