Waymire J C, Weiner N, Prasad K N
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2241-5. doi: 10.1073/pnas.69.8.2241.
Mouse neuroblastoma cells in culture have been used as a model for the study of the mechanism by which activities of tyrosine hydroxylase (EC 1.14.3.a) are regulated in sympathetic tissue. The activity of tyrosine hydroxylase in cultured cells drops to barely detectable activities after 1 week and remains low for months in culture in the uncloned cell line of neuroblastoma. Activity in an adrenergic clone isolated from the uncloned line has about 20% of the activity of the fresh grated tumor cell. N(6), O(2')-dibutyryl adenosine 3':5'-cyclic monophosphate causes a concentration and time-dependent increase in enzyme activity in both the cloned and uncloned cell lines. Enzyme activity is elevated by other stable analogs of adenosine 3':5'-cyclic monophosphate, notably the N(6)-monobutyryl, 8-aminomethyl, and 8-methylthio derivatives of the cyclic nucleotide; by the inhibitor of cyclic nucleotide phosphodiesterase, papaverine; and by sodium butyrate. Changes in cell morphology and tyrosine hydroxylase activity are shown not to be necessarily related.
培养的小鼠神经母细胞瘤细胞已被用作研究交感组织中酪氨酸羟化酶（EC 1.14.3.a）活性调节机制的模型。在未克隆的神经母细胞瘤细胞系中培养1周后，培养细胞中酪氨酸羟化酶的活性降至几乎检测不到的水平，并且在数月的培养过程中一直保持较低水平。从未克隆细胞系中分离出的一个肾上腺素能克隆中的活性约为新鲜磨碎的肿瘤细胞活性的20%。N(6), O(2')-二丁酰腺苷3':5'-环磷酸腺苷在克隆和未克隆细胞系中均导致酶活性呈浓度和时间依赖性增加。腺苷3':5'-环磷酸腺苷的其他稳定类似物，特别是环核苷酸的N(6)-单丁酰、8-氨基甲基和8-甲硫基衍生物；环核苷酸磷酸二酯酶抑制剂罂粟碱；以及丁酸钠，均可提高酶活性。细胞形态和酪氨酸羟化酶活性的变化并不一定相关。
