Gosden C M, Brock D J
Lancet. 1977 Apr 30;1(8018):919-22. doi: 10.1016/s0140-6736(77)92221-8.
In 20 amniotic-fluid samples taken in the second trimester from pregnancies in which the fetus had a neural-tube defect, the proportion of the total viable cell which adhered to glass surfaces after 20 hours in culture ranged from 9 to 100%. In 92 normal amniotic fluids this proportion was less than 6%. Furthermore, the morphology of the rapidly adhering cells was characteristic in spina bifida (8 cases) and anencephaly (12 cases) and distinct from the epithelioid-like cell seen in normal amniotic fluids, including many which were grossly blood-stained. A sample of amniotic fluid from fetal exomphalos and one from a pregnancy in which the placenta had been repeatedly traversed during amniocentesis had proportions of adherent cells in the pathological range, but the morphologies were very different from those seen in neural-tube defects. It is suggested that the techniques described here will be useful adjuncts to amniotic-fluid alpha-fetoprotein determination in the early diagnosis of fetal abnormality, particularly in blood-stained samples.
在妊娠中期采集的20份羊水样本中，胎儿患有神经管缺陷，培养20小时后附着于玻璃表面的活细胞总数比例在9%至100%之间。在92份正常羊水中，这一比例低于6%。此外，快速附着细胞的形态在脊柱裂（8例）和无脑儿（12例）中具有特征性，与正常羊水中所见的上皮样细胞不同，包括许多严重血染的细胞。来自胎儿脐膨出的一份羊水样本以及一份在羊膜穿刺术期间胎盘被反复穿过的妊娠的羊水样本，其附着细胞比例在病理范围内，但形态与神经管缺陷中所见的非常不同。建议这里描述的技术将有助于在胎儿异常的早期诊断中辅助羊水甲胎蛋白测定，特别是在血染样本中。
