在干扰素作用于培养细胞的过程中，转运核糖核酸甲基化是否发生改变或增加？

Altered or increased transfer-RNA methylation in the course of Interferon action on cells in culture?

作者信息

Koblet H, Wyler R, Kohler U

出版信息

Experientia. 1979 May 15;35(5):576-8. doi: 10.1007/BF01960327.

DOI:10.1007/BF01960327

Abstract

The induction of the antiviral state by Interferon might reflect the decrease of the rate of biosynthesis, the degradation or the alteration of one or several tRNAs. This could result in rate-limiting concentrations for codons common in viral RNA but rare in host mRNA. Altered methylation of tRNA could be the basis of such a phenomenon. However, we could not find an altered extent of methylation of total tRNA or an altered pattern of methylation, if mixed tRNAs were chromatographed on MAK- or BD-cellulose columns, despite a large range of conditions of pretreatment of chick embryo fibroblast cultures with interferon.

摘要

干扰素诱导抗病毒状态可能反映了生物合成速率的降低、一种或几种转运RNA（tRNA）的降解或改变。这可能导致病毒RNA中常见但宿主mRNA中罕见的密码子出现限速浓度。tRNA甲基化的改变可能是这种现象的基础。然而，尽管用干扰素对鸡胚成纤维细胞培养物进行了大范围的预处理，但如果将混合tRNA在MAK或BD纤维素柱上进行层析，我们并未发现总tRNA甲基化程度的改变或甲基化模式的改变。