Badaracco G, Laquidara M, Cassani G
Nucleic Acids Res. 1978 Jul;5(7):2577-86. doi: 10.1093/nar/5.7.2577.
Kluyveromyces lactis cells permeabilized with nystatin, though no longer viable, were able to incorporate 3H-dATP into DNA. Maximum rate of synthesis was obtained when all four deoxyribonucleoside triphosphates were present. For prolonged incorporation of 3H-dATP into DNA rATP or phosphoenolpyruvate were of absolute requirement. DNA synthesis was inhibited by p-chloromercuribenzoate, N-ethylmaleimide, nalidixate, ethidium bromide and distamycin A. The density of DNA synthesized in permeabilized cells grown on non-fermentable and fermentable carbon sources was analyzed on CsCl gradients in the presence or absence of distamycin A. The DNA synthesized by permeabilized cells previously grown on glycerol was essentially mitochondrial DNA; nuclear DNA (30% of total) was also synthesized by cells previously grown on glucose.
用制霉菌素通透处理的乳酸克鲁维酵母细胞虽已不再存活，但仍能将3H - dATP掺入DNA中。当所有四种脱氧核糖核苷三磷酸都存在时，可获得最大合成速率。为了使3H - dATP长时间掺入DNA，rATP或磷酸烯醇丙酮酸是绝对必需的。DNA合成受到对氯汞苯甲酸、N - 乙基马来酰亚胺、萘啶酸、溴化乙锭和偏端霉素A的抑制。在存在或不存在偏端霉素A的情况下，在CsCl梯度上分析了在不可发酵和可发酵碳源上生长的通透细胞中合成的DNA的密度。先前在甘油上生长的通透细胞合成的DNA基本上是线粒体DNA；先前在葡萄糖上生长的细胞也合成核DNA（占总量的30%）。
