阿非科林对酵母脱氧核糖核酸聚合酶的作用效果及作用机制
Effect and mechanism of action of aphidicolin on yeast deoxyribonucleic acid polymerases.
作者信息
Plevani P, Badaracco G, Ginelli E, Sora S
出版信息
Antimicrob Agents Chemother. 1980 Jul;18(1):50-7. doi: 10.1128/AAC.18.1.50.
Abstract
The antibiotic aphidicolin inhibited in vitro deoxyribonucleic acid synthesis catalyzed by crude yeast extracts and by partially purified yeast deoxyribonucleic acid polymerases. The mechanism of action of aphidicolin on yeast deoxyribonucleic acid polymerase I was noncompetitive with deoxyguanosine 5'-triphosphate, deoxyadenosine 5'-triphosphate, and deoxythymidine 5'-triphosphate and was of the mixed type with deoxycytidine 5'-triphosphate. The relative ratio of enzyme to the template-initiator complex was important for detecting the inhibitory effect of the antibiotic. The inhibition of in vitro deoxyribonucleic acid synthesis by aphidicolin was reversible, and the effect on yeast deoxyribonucleic acid polymerase might have been partially mediated by some supplementary factor(s).
摘要
抗生素阿非科林可抑制粗制酵母提取物和部分纯化的酵母脱氧核糖核酸聚合酶催化的体外脱氧核糖核酸合成。阿非科林对酵母脱氧核糖核酸聚合酶I的作用机制，与脱氧鸟苷5'-三磷酸、脱氧腺苷5'-三磷酸和脱氧胸苷5'-三磷酸呈非竞争性，与脱氧胞苷5'-三磷酸呈混合型。酶与模板-引发剂复合物的相对比例，对于检测该抗生素的抑制作用很重要。阿非科林对体外脱氧核糖核酸合成的抑制作用是可逆的，其对酵母脱氧核糖核酸聚合酶的影响可能部分由某些辅助因子介导。
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Low molecular weight deoxyribonucleic acid polymerase from calf thymus chromatin. I. Preparation of homogeneous enzyme.来自小牛胸腺染色质的低分子量脱氧核糖核酸聚合酶。I. 纯酶的制备。
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Antiviral effects of aphidicolin, a new antibiotic produced by Cephalosporium aphidicola.头孢蚜霉菌产生的一种新型抗生素——阿非科林的抗病毒作用
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Variations of DNA polymerase-alpha and -beta during prolonged stimulation of human lymphocytes.人淋巴细胞长期刺激过程中DNA聚合酶α和β的变化
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RNA polymerase from phage SP01-infected and uninfected Bacillus subtilis.来自噬菌体SP01感染和未感染的枯草芽孢杆菌的RNA聚合酶。
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