在制霉菌素处理的乳酸克鲁维酵母细胞中依赖外源三磷酸的核糖核酸合成
Ribonucleic acid synthesis dependent on exogenous triphosphates in nystatin-treated cells of of Kluyveromyces latis.
作者信息
Badaracco G, Cassani G
出版信息
Antimicrob Agents Chemother. 1976 May;9(5):748-53. doi: 10.1128/AAC.9.5.748.
Abstract
Kluyveromyces lactis cells treated with nystatin became permeable to ribonucleoside triphosphates. Although not viable, nystatin-treated cells were capable of sustaining ribonucleic acid synthesis. The system depended on the presence of divalent ions and the four nucleoside triphosphates, and was strongly stimulated by ammonium sulfate. The system utilized endogenous deoxyribonucleic acid as template. Synthesis of ribonucleic acid was associated with the cells and probably occurred internally.
摘要
用制霉菌素处理的乳酸克鲁维酵母细胞对三磷酸核糖核苷变得具有通透性。尽管经制霉菌素处理的细胞无法存活，但它们仍能够维持核糖核酸的合成。该系统依赖于二价离子和四种三磷酸核苷的存在，并受到硫酸铵的强烈刺激。该系统利用内源性脱氧核糖核酸作为模板。核糖核酸的合成与细胞相关联，可能发生在细胞内部。
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