Hrabac B, Catović D, Nikulin A
Neoplasma. 1984;31(3):299-305.
For estimation of the value of Chemetron method for LD isoenzyme separation on cellulose-acetate membranes in cancer tissues taken from corpses, malignant tumors revealed by autopsy were investigated. Overall stability of LD, as well as the maintenance of various forms of isoenzymes in the extracts with regard to post-mortem autolysis in corpse and long periods of freezing, have been demonstrated. No correlation was found between the degree of isoenzyme changes and the histologic grading of tumors. LD isoenzyme pattern in cancer metastasis has been considered with respect to vascular and metabolic role of the environmental normal tissue. In order to point out the possible role of LD isoenzyme pattern in cancer diagnosis a lot of preliminary studies has been reviewed. The clarification of the specific distribution of LD activities in different tissues has been considered.
为评估Chemetron法在从尸体获取的癌组织中于醋酸纤维素膜上分离乳酸脱氢酶（LD）同工酶的价值，对尸检发现的恶性肿瘤进行了研究。已证实LD的总体稳定性，以及提取物中各种同工酶形式在尸体死后自溶和长期冷冻方面的维持情况。未发现同工酶变化程度与肿瘤组织学分级之间存在相关性。已从周围正常组织的血管和代谢作用方面考虑了癌转移中的LD同工酶模式。为指出LD同工酶模式在癌症诊断中的可能作用，回顾了许多初步研究。已考虑阐明LD活性在不同组织中的具体分布情况。
