Sexton T J, Fewlass T A
Poult Sci. 1978 Jan;57(1):277-84. doi: 10.3382/ps.0570277.
The major objective of this study was to assess the relationship of various components of the Beltsville Poultry Semen Extender (BPSE) to the maintenance of the fertilizing capacity of chicken semen stored at 5 degrees C. A secondary objective was to rest the effectiveness of certain chemical additives on maintining the fertilizing capacity of stored semen. The data indicate that the components of the BPSE which contribute most to the preservation of semen were sodium glutamate and potassium phosphate. Also the high level of potassium in the BPSE was critical to the survival of spermatozoa during low temperature storage. Although the fructose component of the BPSE was not a significant factor during storage, attempts to substitute fructose with other carbohydrates resulted in a reduction in fertility. The addition of gentamicin sulfate (100 to 200 microgram/ml.), or pasteurized milk (10% v./v.) to the BPSE did not significantly alter the fertilizing capacity of semen stored for 24 hr., whereas similar levels of whole egg, egg yolk, egg albumen or seminal plasma had a depressive effect. The data in this study clearly indicate that components of and additions to the BPSE were responsible for the maintenance of only about 50 to 60% of the fertilizing capacity of chicken semen stored for 24 hr. at 5 degrees C.
本研究的主要目的是评估贝尔茨维尔家禽精液稀释液（BPSE）的各种成分与在5摄氏度下储存的鸡精液受精能力维持之间的关系。次要目的是检验某些化学添加剂对维持储存精液受精能力的有效性。数据表明，对精液保存贡献最大的BPSE成分是谷氨酸钠和磷酸钾。此外，BPSE中的高钾水平对于低温储存期间精子的存活至关重要。尽管BPSE中的果糖成分在储存期间不是一个重要因素，但用其他碳水化合物替代果糖会导致受精率下降。向BPSE中添加硫酸庆大霉素（100至200微克/毫升）或巴氏杀菌牛奶（10% v/v）不会显著改变储存24小时的精液的受精能力，而类似水平的全蛋、蛋黄、蛋清或精浆则有抑制作用。本研究中的数据清楚地表明，BPSE的成分和添加物仅负责维持在5摄氏度下储存24小时的鸡精液约50%至60%的受精能力。
