Howarth B
Poult Sci. 1983 Jun;62(6):1084-7. doi: 10.3382/ps.0621084.
Beltsville Poultry Semen Extender (BPSE) and Lake's Diluent A (LDA) were compared with minimum essential medium (MEM) for their ability to maintain the fertilizing capacity of cock semen held 6 hr at 41 C. Motility significantly declined from the beginning to the end of the holding period for semen in BPSE and LDA. Only in LDA, however, were the number of live spermatozoa significantly reduced. Although there were no differences in oxygen (O2) consumption measured at 1 and 6 hr for semen in BPSE and MEM, a significant reduction in O2 consumption was observed between these time periods for semen in LDA. Fertility of semen held in MEM (90.3%) was significantly higher than the unstored control semen (82.9%) and semen held in either BPSE (3.5%) or LDA (1.9%). No differences in hatchability of fertile eggs were observed between the semen groups.
将贝尔茨维尔家禽精液稀释剂(BPSE)和莱克稀释液A(LDA)与最低限度基本培养基(MEM)进行比较,以评估它们在41℃下保存6小时时维持公鸡精液受精能力的能力。在BPSE和LDA中,精液在保存期开始到结束时活力显著下降。然而,只有在LDA中,活精子数量才显著减少。虽然在1小时和6小时时测量的BPSE和MEM中精液的氧气(O₂)消耗量没有差异,但在LDA中精液的这些时间段之间观察到O₂消耗量显著降低。保存在MEM中的精液受精率(90.3%)显著高于未保存的对照精液(82.9%)以及保存在BPSE(3.5%)或LDA(1.9%)中的精液。精液组之间在受精卵孵化率上没有观察到差异。