Thurston R J, Rogoff M S, Scott T R, Korn N
Department of Poultry Science, Clemson University, South Carolina 29634.
Poult Sci. 1993 Mar;72(3):598-602. doi: 10.3382/ps.0720598.
The perfluorochemicals (PFC) have a high capacity for dissolving oxygen and carbon dioxide. Two experiments were conducted using PFC as diluent components to assess their effect on the fertilizing capacity of, and the hatchability of eggs from hens inseminated with, turkey semen stored for 30 min or 3 h. Two PFC were tested for the 30-min experiment: perfluorohexane (FC-72) and perfluorobutyltetrahydrofurane (FC-80). For the 3-h experiment, these PFC plus bis-1,2-(F-butyl)-ethene (F-44E) were studied. The PFC were emulsified with an equal volume of a standard aqueous salt diluent, Beltsville Poultry Semen Extender (BPSE). The BPSE alone was used as a control in both experiments. Semen was diluted 1:2 with oxygen-saturated extender and stored in closed tubes at 20 to 30 C prior to insemination. For the 30-min study, PFC diluents resulted in fertility rates comparable to (FC-72) or significantly greater than (FC-80) BPSE alone (P < or = .05). There was no significant difference in hatchability due to any semen treatment. When semen was stored for 3 h, PFC diluents resulted in comparable (FC-80) or significantly higher (F-44E and FC-72) fertility and hatchability rates. Significantly lower embryonic deaths at Day 10 of incubation were noted for all PFC diluents. These findings provide evidence that PFC emulsions are not toxic and prolong fertilizing capacity of turkey sperm during short-term storage, and suggest that providing oxygen carriers such as PFC in semen diluents may be beneficial.
全氟化合物（PFC）具有很高的溶解氧气和二氧化碳的能力。进行了两项实验，使用PFC作为稀释剂成分，以评估它们对用储存30分钟或3小时的火鸡精液人工授精的母鸡的受精能力和孵化率的影响。在30分钟的实验中测试了两种PFC：全氟己烷（FC-72）和全氟丁基四氢呋喃（FC-80）。在3小时的实验中，研究了这些PFC加上双-1,2-（氟丁基）-乙烯（F-44E）。将PFC与等体积的标准盐水稀释剂——贝尔茨维尔家禽精液稀释剂（BPSE）乳化。在两个实验中均单独使用BPSE作为对照。精液用饱和氧气的稀释剂以1:2的比例稀释，并在授精前于20至30℃下储存在封闭管中。在30分钟的研究中，PFC稀释剂导致的受精率与单独使用BPSE相当（FC-72）或显著高于（FC-80）（P≤0.05）。由于任何精液处理导致的孵化率没有显著差异。当精液储存3小时时，PFC稀释剂导致的受精率相当（FC-80）或显著更高（F-44E和FC-72），孵化率也更高。在孵化第10天，所有PFC稀释剂的胚胎死亡率均显著降低。这些发现提供了证据，表明PFC乳液无毒，并且在短期储存期间可延长火鸡精子的受精能力，还表明在精液稀释剂中提供诸如PFC的氧气载体可能是有益的。
