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多杀巴斯德氏菌克莱姆森大学菌株新鲜疫苗培养物的生长曲线及稳定性

Growth curves and stabilization of fresh vaccine cultures of the Clemson University strain of Pastuerella multocida.

作者信息

Jensen M M

出版信息

Poult Sci. 1978 May;57(3):593-5. doi: 10.3382/ps.0570593.

DOI:10.3382/ps.0570593
PMID:674040
Abstract

Growth curves at 37 C for the Clemson University strain of Pasteurella multocida were about the same in either brain-heart-infusion broth or in a recently developed broth called "special broth TV". Counts in both broths were usually greater than 1 x 10(9) viable bacteria per ml. With the addition of 5 g of dried skim milk to each 100 ml of broth culture in the stationary phase of the growth curve, only a slight decrease in viable counts occurred over the next 7 to 14 days when held at 4 C. Such stabilization of broth cultures of P. multocida gives added flexibility to the use of fresh cultures as a live oral turkey cholera vaccine.

摘要

多杀巴斯德氏菌克莱姆森大学菌株在37℃下于脑心浸液肉汤或一种新开发的名为“特殊肉汤TV”的肉汤中的生长曲线大致相同。两种肉汤中的菌数通常都大于每毫升1×10⁹个活菌。在生长曲线的稳定期,向每100毫升肉汤培养物中添加5克脱脂奶粉后,在4℃保存的接下来7至14天里,活菌数仅略有下降。多杀巴斯德氏菌肉汤培养物的这种稳定性为将新鲜培养物用作活口服火鸡霍乱疫苗的使用提供了更大的灵活性。

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