Rose G A, Sulaiman S
Urol Int. 1984;39(3):147-9. doi: 10.1159/000280963.
Macromolecules were removed from whole urine samples by sequential ultrafiltration using molecular weight cut-offs of 300,000, 100,000 and 10,000 dalton. The whole urine samples and ultrafiltrates were evaporated to 1,250 mosm/kg at pH 5.3 and 6.8 to study calcium oxalate and calcium phosphate crystal formation, respectively. While all the ultrafiltrates removed Tamm-Horsfall proteins and most of the crystal formation, there was some 10% of the crystal-promoting powers in the macromolecules in the fraction between molecular weight 100,000 and 300,000 dalton. This demonstrates the existence of a second crystal-promoting substance in the macromolecules of whole urine.
通过使用截留分子量为300,000、100,000和10,000道尔顿的超滤膜对全尿样本进行连续超滤，去除大分子物质。将全尿样本和超滤液分别在pH值为5.3和6.8的条件下蒸发至1,250 mosm/kg，以研究草酸钙和磷酸钙晶体的形成。虽然所有超滤液都去除了Tamm-Horsfall蛋白并减少了大部分晶体形成，但在分子量介于100,000和300,000道尔顿之间的大分子部分中，仍有大约10%的促进晶体形成的能力。这证明了全尿大分子中存在第二种促进晶体形成的物质。
