Oborotova T A, Berman A E, Mazurov V I
Biokhimiia. 1980 Mar;45(3):554-60.
The quantitative ratio, chemical composition and sedimentation properties of three polyribosomal fractions (free polyribosomes and polyribosomes tightly and loosely bound to the endoplasmic reticulum) isolated from chicken embryos of different age were studied. The fraction of polyribosomes loosely bound to the membranes was obtained from the total preparation of membrane-bound polyribosomes by treatment with high ionic strength solutions. It was found that the polyribosomes loosely bound to the membranes and free polyribosomes possess similar chemical composition and sedimentation properties. All the fractions studied were characterized by a much higher polyribosomal content as compared to the 80S monomers. In the course of embryogenesis the amount of polyribosomes loosely bound to the membranes is decreased, whereas that of tightly bound ones is increased. These data are indicative of an enhanced synthesis of secreted proteins, particularly collagen, on tightly bound polyribosomes during embryogenesis in chicken.
对从不同年龄鸡胚中分离出的三种多核糖体组分（游离多核糖体以及紧密和松散结合在内质网上的多核糖体）的定量比率、化学组成和沉降特性进行了研究。通过用高离子强度溶液处理，从膜结合多核糖体的总制剂中获得了松散结合在膜上的多核糖体组分。发现松散结合在膜上的多核糖体和游离多核糖体具有相似的化学组成和沉降特性。与80S单体相比，所有研究的组分都具有更高的多核糖体含量。在胚胎发生过程中，松散结合在膜上的多核糖体数量减少，而紧密结合的多核糖体数量增加。这些数据表明，在鸡胚胎发生过程中，紧密结合的多核糖体上分泌蛋白尤其是胶原蛋白的合成增强。
