Nash P V, Bjornsson T D
Anal Biochem. 1984 May 1;138(2):319-23. doi: 10.1016/0003-2697(84)90815-7.
A method for determining heparin activity (in unit/ml) in human plasma is described. The method is based on neutralization of heparin by Polybrene , a polymerized quaternary ammonium salt. It uses serial incubations of plasma with increasing amounts of Polybrene in conjunction with thrombin-induced coagulation times to define the amount of Polybrene required to neutralize heparin in the sample. Reference curves involve linear relationships between amounts of Polybrene required to neutralize known amounts of heparin. Coefficients of variation for the assay vary from 4 to 10% over the range of 0.05 to 1.0 unit/ml.
本文描述了一种测定人血浆中肝素活性（单位/毫升）的方法。该方法基于用鱼精蛋白（一种聚合季铵盐）中和肝素。它利用血浆与逐渐增加量的鱼精蛋白进行系列孵育，并结合凝血酶诱导的凝血时间来确定中和样品中肝素所需的鱼精蛋白量。参考曲线涉及中和已知量肝素所需鱼精蛋白量之间的线性关系。该测定的变异系数在0.05至1.0单位/毫升范围内为4%至10%。
