Russell R W, Macri J
Pharmacol Biochem Behav. 1978 Apr;8(4):399-403. doi: 10.1016/0091-3057(78)90076-x.
Evidence supports the proposition that the high affinity Ch uptake system associated with cholinergic nerve terminals can be the rate limiting step in the synthesis of the neurotransmitter, acetylcholine. The present experiment was designed to test the hypothesis that variations of the system would be reflected selectively in changes of conditioned avoidance behavior. HC-3, which primarily affects the high affinity component of Ch transport thus reducing endogenous levels of ACh and the synthetic capacity of cholinergic nerve terminals, was administered cerebroventricularly at 5 doses ranging from 0.0 (saline control) to 10.0 microgram. Whole brain ACh levels determined by GCMS analysis following microwave fixation ranged from 25.0 to 5.0 nmol/g-1. Trend analyses demonstrate a precise dose dependent relation between neurochemical and behavioral variables: median trials to condition increased as ACh level decreased. More detailed analyses of the results lead to the interpretation that suppression of high affinity Ch transport in brain is associated with deficiencies in the use of information and not with sensory input and storage nor with motor output.
有证据支持这样的观点，即与胆碱能神经末梢相关的高亲和力胆碱摄取系统可能是神经递质乙酰胆碱合成中的限速步骤。本实验旨在检验以下假设：该系统的变化会选择性地反映在条件性回避行为的改变中。HC - 3主要影响胆碱转运的高亲和力成分，从而降低乙酰胆碱的内源性水平和胆碱能神经末梢的合成能力，通过脑室内注射给予5种剂量，范围从0.0（生理盐水对照）到10.0微克。在微波固定后通过气相色谱 - 质谱分析测定的全脑乙酰胆碱水平在25.0至5.0 nmol/g - 1之间。趋势分析表明神经化学和行为变量之间存在精确的剂量依赖关系：随着乙酰胆碱水平降低，条件化的中位试验次数增加。对结果进行更详细的分析得出的解释是，脑中高亲和力胆碱转运的抑制与信息利用不足有关，而与感觉输入和存储以及运动输出无关。
