Crowe J H, Crowe L M, Chapman D
Arch Biochem Biophys. 1984 Jul;232(1):400-7. doi: 10.1016/0003-9861(84)90555-1.
Infrared spectroscopy was used to investigate the changes in bands assigned to phospholipids and proteins in dehydrated and rehydrated sarcoplasmic reticulum. The changes in CH2 and CH3 stretching bands, amide bands, and phosphate stretching bands are similar to shifts in frequency seen for those bands in phospholipid and protein preparations during thermotropic phase transitions and hydration. IR studies on dry trehalose-sarcoplasmic reticulum mixtures show similar results; with increasing trehalose concentration in the dry mixtures, amide and phosphate bands shift to frequencies characteristic of hydrated samples. Changes in bands assigned to OH deformations in the trehalose suggest that the interaction between the carbohydrate and membrane is by means of hydrogen bonding between these -OH groups and membrane components.
利用红外光谱研究脱水和复水后的肌质网中磷脂和蛋白质相关谱带的变化。CH2和CH3伸缩谱带、酰胺谱带以及磷酸伸缩谱带的变化，与磷脂和蛋白质制剂在热致相变和水合过程中这些谱带的频率变化相似。对干燥的海藻糖-肌质网混合物进行的红外研究显示了类似的结果；随着干燥混合物中海藻糖浓度的增加，酰胺和磷酸谱带移向水合样品特有的频率。海藻糖中与OH变形相关谱带的变化表明，碳水化合物与膜之间的相互作用是通过这些-OH基团与膜成分之间的氢键实现的。
