El-On J, Messer G, Greenblatt C L
Ann Trop Med Parasitol. 1984 Apr;78(2):93-8. doi: 10.1080/00034983.1984.11811782.
Rifampicin and Amphotericin B (AMB) were studied separately and in combination to determine their effect in vitro on intracellular Leishmania tropica amastigotes. On the fourth day of treatment, rifampicin at 200 micrograms ml-1 or AMB at 0.15 microgram ml-1 reduced the parasites' survival index (PSI) to 10% compared with the untreated control. In combination, there was a significant synergistic effect at several concentrations. The synergistic therapeutic effects were not accompanied by toxicity to the host cells, although each drug alone caused cellular degeneration in the ultrastructure of the parasites with the formation of large autophagic-like vacuoles, while the combination increased the percentage of degenerate cells.
分别对利福平与两性霉素B（AMB）及其联合用药进行了研究，以确定它们在体外对热带利什曼原虫无鞭毛体的作用。在治疗的第四天，与未处理的对照组相比，200微克/毫升的利福平或0.15微克/毫升的AMB将寄生虫存活指数（PSI）降低至10%。联合用药时，在几个浓度下均有显著的协同作用。尽管每种药物单独使用时都会导致寄生虫超微结构中的细胞变性，形成大型自噬样空泡，但联合用药时这种协同治疗效果并未伴随对宿主细胞的毒性，而联合用药增加了变性细胞的百分比。
