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Isolation and partial characterization of plasmid DNA from Arthrobacter oxidans.

作者信息

Brandsch R, Decker K

出版信息

Arch Microbiol. 1984 May;138(1):15-7. doi: 10.1007/BF00425400.

DOI:10.1007/BF00425400
PMID:6742952
Abstract

A method for the extraction of the high molecular weight plasmid AO 1 from the gram-positive soil bacterium Arthrobacter oxidans is presented. Following digestion of this DNA with the restriction endonucleases AccI, Bam HI, Eco RI and Hind III, an average molecular mass of 157.8 kb was estimated. This value is in good agreement with the 160 kb size determined previously by electron microscopy (Brandsch et al. 1982). Using the same method, no plasmid DNA was found in strains of the genus Arthrobacter which do not degrade nicotine, e.g., A. albidus, A. globiformis and A. auricans.

摘要

相似文献

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Isolation and partial characterization of plasmid DNA from Arthrobacter oxidans.
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本文引用的文献

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一个编码6-羟基-3-琥珀酰吡啶羟化酶的新基因，参与恶臭假单胞菌S16菌株对尼古丁的降解。
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Characterization of PmfR, the transcriptional activator of the pAO1-borne purU-mabO-folD operon of Arthrobacter nicotinovorans.嗜烟节杆菌pAO1携带的purU-mabO-folD操纵子的转录激活因子PmfR的特性分析
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Sequence of the 165-kilobase catabolic plasmid pAO1 from Arthrobacter nicotinovorans and identification of a pAO1-dependent nicotine uptake system.来自嗜烟节杆菌的165千碱基分解代谢质粒pAO1的序列及一种依赖pAO1的尼古丁摄取系统的鉴定。
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Gene cluster on pAO1 of Arthrobacter nicotinovorans involved in degradation of the plant alkaloid nicotine: cloning, purification, and characterization of 2,6-dihydroxypyridine 3-hydroxylase.嗜烟节杆菌pAO1上参与植物生物碱尼古丁降解的基因簇：2,6 - 二羟基吡啶3 - 羟化酶的克隆、纯化及特性分析
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A pAO1-encoded molybdopterin cofactor gene (moaA) of Arthrobacter nicotinovorans: characterization and site-directed mutagenesis of the encoded protein.嗜烟节杆菌的一个由pAO1编码的钼蝶呤辅因子基因（moaA）：编码蛋白的特性及定点诱变
Arch Microbiol. 1995 Aug;164(2):142-51. doi: 10.1007/BF02525320.
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Degradation and induction specificity in actinomycetes that degrade p-nitrophenol.降解对硝基苯酚的放线菌中的降解及诱导特异性
Appl Environ Microbiol. 1993 Oct;59(10):3505-8. doi: 10.1128/aem.59.10.3505-3508.1993.
9
Isolation and characterization of an s-ethyl-N,N-dipropylthiocarbamate-degrading Arthrobacter strain and evidence for plasmid-associated s-ethyl-N,N-dipropylthiocarbamate degradation.一株降解仲草丹的节杆菌菌株的分离与鉴定及与质粒相关的仲草丹降解证据
Appl Environ Microbiol. 1987 May;53(5):1088-93. doi: 10.1128/aem.53.5.1088-1093.1987.
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Plasmid pAO1 of Arthrobacter oxidans encodes 6-hydroxy-D-nicotine oxidase: cloning and expression of the gene in Escherichia coli.氧化节杆菌的质粒pAO1编码6-羟基-D-尼古丁氧化酶：该基因在大肠杆菌中的克隆与表达。
Mol Gen Genet. 1986 Jan;202(1):96-101. doi: 10.1007/BF00330523.
质粒在多氯联苯完全降解中的作用。
Appl Environ Microbiol. 1982 Sep;44(3):619-26. doi: 10.1128/aem.44.3.619-626.1982.
4
Rapid methods for the study of both stable and unstable plasmids in Pseudomonas.研究假单胞菌中稳定和不稳定质粒的快速方法。
J Gen Microbiol. 1981 Jun;124(2):433-7. doi: 10.1099/00221287-124-2-433.
5
A rapid boiling method for the preparation of bacterial plasmids.一种制备细菌质粒的快速煮沸法。
Anal Biochem. 1981 Jun;114(1):193-7. doi: 10.1016/0003-2697(81)90473-5.
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Rapid purification of covalently closed circular DNAs of bacterial plasmids and animal tumor viruses.细菌质粒和动物肿瘤病毒共价闭合环状DNA的快速纯化
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Nucleic Acids Res. 1979 Nov 24;7(6):1513-23. doi: 10.1093/nar/7.6.1513.