Liu D K, Owens G F
Biochem Biophys Res Commun. 1984 Jun 29;121(3):788-95. doi: 10.1016/0006-291x(84)90747-2.
Treatment of rats with phenobarbital for three days greatly increases the activity of 2,5 oligoadenylate synthetase in liver nuclei. Analysis of 2',5'-oligoadenylates synthesized in vitro showed that nuclei from both phenobarbital-treated and control rats synthesized 2',5'-oligoadenylates ranging from di- to hexamers. However, nuclei from drug treated rats showed a two fold increase in trimer and tetramer synthesis and a three-four fold increase in longer chained oligoadenylates. There was no change in the nuclear 2'-phosphodiesterase activity as the result of phenobarbital treatment, This activity remained low in nuclei from either the treated or the control rats. To our knowledge, this is the first report on phenobarbital affecting the liver 2',5'-oligoadenylate system.
用苯巴比妥处理大鼠三天可显著提高肝细胞核中2,5-寡腺苷酸合成酶的活性。对体外合成的2',5'-寡腺苷酸的分析表明，来自苯巴比妥处理组和对照组大鼠的细胞核均能合成二聚体至六聚体的2',5'-寡腺苷酸。然而，药物处理组大鼠的细胞核三聚体和四聚体合成增加了两倍，长链寡腺苷酸合成增加了三到四倍。苯巴比妥处理并未改变细胞核2'-磷酸二酯酶的活性，该活性在处理组或对照组大鼠的细胞核中均保持较低水平。据我们所知，这是关于苯巴比妥影响肝脏2',5'-寡腺苷酸系统的首次报道。
