Kaliman P A, Nam S F
Ukr Biokhim Zh (1978). 1984 Nov-Dec;56(6):619-24.
DNA-dependent RNA-polymerases of nuclei isolated from the liver of rats aged 3- and 24 months were studied in different periods after phenobarbital administration. It is shown that 4h after single intraperitoneal injection of the preparation (80 mg per 1 kg of the body mass) to young animals the activity of the RNA-polymerase I rises, it remains higher, the following 12-20 h and then returns to the initial level. The activity of RNA-polymerases II and III under these conditions 16 h later remains higher the following 8 h and 24 h later the activity of the first enzyme returns to the initial value and that of the second one becomes still more. In old rats (24 months) the activity of all three classes of RNA-polymerases is lower than in young ones and increases only 48 h later. Under long-term administration of phenobarbital (40 mg per 1 kg of the body mass daily, 4 days) the activity of RNA-polymerase I was the same as in the intact animals of the corresponding ages and the activity of RNA-polymerases II and III increased both in young and old rats. Single administration of the preparation increases the liver mass in the young animals 24 h later and in the old ones--48 h later. Long-term administration of the preparation enhances its mass both in young and old animals, but its relative increase is more expressed in rats at the age of 3 months.
对3个月和24个月大的大鼠肝脏分离出的细胞核中的DNA依赖性RNA聚合酶，在给予苯巴比妥后的不同时间段进行了研究。结果表明，对幼龄动物单次腹腔注射该制剂（每1千克体重80毫克）4小时后，RNA聚合酶I的活性升高，在接下来的12 - 20小时内保持较高水平，然后恢复到初始水平。在这些条件下，16小时后RNA聚合酶II和III的活性在接下来的8小时内保持较高，24小时后第一种酶的活性恢复到初始值，而第二种酶的活性变得更高。在老龄大鼠（24个月）中，所有三类RNA聚合酶的活性均低于幼龄大鼠，且仅在48小时后增加。长期给予苯巴比妥（每天每1千克体重40毫克，共4天），RNA聚合酶I的活性与相应年龄的完整动物相同，RNA聚合酶II和III的活性在幼龄和老龄大鼠中均增加。单次给予该制剂24小时后可增加幼龄动物的肝脏质量，48小时后增加老龄动物的肝脏质量。长期给予该制剂可增加幼龄和老龄动物的肝脏质量，但其相对增加在3个月龄的大鼠中更为明显。
