一种用于纯化菠菜类囊体中水溶性33千道尔顿蛋白质的快速、高产方法。
A rapid, high-yield method for the purification of the water-soluble 33 kilodalton protein of spinach thylakoids.
作者信息
Franzén L G, Andréasson L E
出版信息
Biochem Biophys Res Commun. 1984 Jul 18;122(1):17-20. doi: 10.1016/0006-291x(84)90432-7.
PMID:6743328
Abstract
A new purification procedure for the water-soluble 33 kDa protein of Photosystem II is presented. The method is based on the selective release of the 33 kDa protein at slightly elevated temperatures and involves a minimum of purification steps. Starting with spinach leaves, the pure protein may be obtained in about 4 h, with a yield usually higher than 60%.
摘要
本文介绍了一种用于光系统II水溶性33 kDa蛋白的新纯化方法。该方法基于在略高温度下选择性释放33 kDa蛋白，且纯化步骤最少。从菠菜叶开始，大约4小时即可获得纯蛋白，产量通常高于60%。
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