叶绿体片层及片层组分多肽组成的比较研究。
Comparative studies on the polypeptide composition of chloroplast lamellae and lamellar fractions.
作者信息
Nolan W G, Park R B
出版信息
Biochim Biophys Acta. 1975 Feb 14;375(3):406-21. doi: 10.1016/0005-2736(75)90356-9.
The polypeptide composition of spinach chloroplast membranes and membrane fractions has been examined by the technique of sodium dodecylsulfate-polyacrylamide gel electrophoresis. Chloroplasts were fragmented into grana (Photosystem II enriched) and stroma lamellae (Photosystem I in character) by the French press technique. The grana lamellae were further fractionated by the use of digitonin into two fractions, one enriched in Photosystem II and the other enriched in Photosystem I. These membranes are composed of at least 15 polypeptides two of which, with approximate weights of 39 and 50 kdaltons, are observed only in granal fractions. Quantitatively the primarily Photosystem II fractions are enriched in polypeptides in the 30-23 kdalton range whereas the Photosystem I (or Photosystem I-enriched) fractions are enriched in polypeptides in the 60-54 kdalton region. The experiments reported show that contamination by soluble proteins or other membranes is negligible. The results indicate that subtle differences in composition account for the large differences in structure and function within the chloroplast membrane system.
已通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳技术对菠菜叶绿体膜及其膜组分的多肽组成进行了检测。通过法国压榨技术将叶绿体破碎成基粒（富含光系统II）和基质类囊体（具有光系统I的特征）。使用洋地黄皂苷将基粒类囊体进一步分离成两个组分，一个富含光系统II，另一个富含光系统I。这些膜由至少15种多肽组成，其中两种多肽的分子量约为39和50千道尔顿，仅在基粒组分中观察到。从数量上看，主要的光系统II组分富含分子量在30 - 23千道尔顿范围内的多肽，而光系统I（或富含光系统I）组分富含分子量在60 - 54千道尔顿区域的多肽。所报道的实验表明，可溶性蛋白质或其他膜的污染可忽略不计。结果表明，组成上的细微差异导致了叶绿体膜系统在结构和功能上的巨大差异。
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