Dunford H B, Baader W J, Bohne C, Cilento G
Biochem Biophys Res Commun. 1984 Jul 18;122(1):28-32. doi: 10.1016/0006-291x(84)90434-0.
The reaction of isobutyraldehyde with dissolved oxygen catalyzed by horseradish peroxidase has been studied from the standpoint of determining the rate-limiting factor under a variety of conditions. Chemiluminescence from the product triplet acetone and rate of oxygen uptake were determined in simultaneous experiments. The reaction is initiated by the peracid obtained from the uncatalyzed autoxidation of the aldehyde; and under certain conditions the amount of peracid is also rate-limiting in the steady-state portion of the reaction. Under other conditions the total amount of enzyme and under still others the rate of formation of enol from the parent aldehyde controls the rate.
从确定各种条件下的限速因素的角度出发，对辣根过氧化物酶催化异丁醛与溶解氧的反应进行了研究。在同步实验中测定了产物三重态丙酮的化学发光和氧气吸收速率。该反应由醛的非催化自氧化产生的过酸引发；在某些条件下，过酸的量在反应的稳态部分也是限速因素。在其他条件下，酶的总量起控制作用，而在另外一些条件下，母体醛形成烯醇的速率起控制作用。
