Berlin E, Shapiro S G, Friedland M
Atherosclerosis. 1984 May-Jun;51(2-3):223-39. doi: 10.1016/0021-9150(84)90170-9.
Aggregation of rabbit platelets from citrated plasma in response to ADP was directly correlated with platelet plasma membrane fluidity as determined by fluorescence depolarization measurements with the probe diphenylhexatriene. Rabbits were maintained for periods of 200 and 400 days on potentially hyperlipidemic diets (20% fat by weight) with varying levels of saturated and polyunsaturated fatty acids. Dietary variations were effective in modulating the mole percentage distribution patterns of the platelet phospholipid fatty acids. The major chemical control of membrane fluidity was the actual mass of unsaturated lipid in the cells and not simply the relative percentage distributions of such unsaturated fatty acids. Substantially higher phospholipid/protein ratios were observed upon analysis of platelets and platelet membranes from rabbits after 200- than after 400-day diet periods. Accordingly lipid structures were significantly more fluid in either whole platelets or membrane isolates at the end of the shorter diet period. The observations pertaining to the extent of aggregation and membrane fluidity are in consonance with the general role of membrane fluidity in controlling biological activity and support the concept that platelet aggregation is a membrane-associated phenomenon.
用探针二苯基己三烯通过荧光去极化测量法测定，枸橼酸盐血浆中兔血小板对ADP的聚集与血小板质膜流动性直接相关。将兔子置于潜在高脂血症饮食（按重量计20%脂肪）中200天和400天，饮食中饱和脂肪酸和多不饱和脂肪酸水平不同。饮食变化有效地调节了血小板磷脂脂肪酸的摩尔百分比分布模式。膜流动性的主要化学控制因素是细胞中不饱和脂质的实际质量，而不仅仅是此类不饱和脂肪酸的相对百分比分布。分析200天饮食期后兔子的血小板和血小板膜时，观察到的磷脂/蛋白质比率显著高于400天饮食期后的比率。因此，在较短饮食期结束时，无论是全血小板还是膜分离物中的脂质结构流动性都明显更高。关于聚集程度和膜流动性的观察结果与膜流动性在控制生物活性中的一般作用一致，并支持血小板聚集是一种膜相关现象的概念。
