通过光学和电子顺磁共振数据的同步非线性分析测量旋转动力学。
Measurement of rotational dynamics by the simultaneous nonlinear analysis of optical and EPR data.
作者信息
Hustedt E J, Cobb C E, Beth A H, Beechem J M
机构信息
Vanderbilt University, Nashville, Tennessee 37232.
出版信息
Biophys J. 1993 Mar;64(3):614-21. doi: 10.1016/S0006-3495(93)81420-6.
Abstract
In the preceding companion article in this issue, an optical dye and a nitroxide radical were combined in a new dual function probe, 5-SLE. In this report, it is demonstrated that time-resolved optical anisotropy and electron paramagnetic resonance (EPR) data can be combined in a single analysis to measure rotational dynamics. Rigid-limit and rotational diffusion models for simulating nitroxide EPR data have been incorporated into a general non-linear least-squares procedure based on the Marquardt-Levenberg algorithm. Simultaneous fits to simulated time-resolved fluorescence anisotropy and linear EPR data, together with simultaneous fits to experimental time-resolved phosphorescence anisotropy decays and saturation transfer EPR (ST-EPR) spectra of 5-SLE noncovalently bound to bovine serum albumin (BSA) have been performed. These results demonstrate that data from optical and EPR experiments can be combined and globally fit to a single dynamic model.
摘要
在本期之前的配套文章中，一种光学染料和一种氮氧化物自由基被组合在一种新型双功能探针5-SLE中。在本报告中，结果表明，时间分辨光学各向异性和电子顺磁共振（EPR）数据可以在单一分析中结合起来以测量旋转动力学。用于模拟氮氧化物EPR数据的刚性极限和旋转扩散模型已被纳入基于马夸特-列文伯格算法的通用非线性最小二乘法程序中。已对模拟的时间分辨荧光各向异性和线性EPR数据进行了同时拟合，并且对与牛血清白蛋白（BSA）非共价结合的5-SLE的实验时间分辨磷光各向异性衰减和饱和转移EPR（ST-EPR）光谱进行了同时拟合。这些结果表明，来自光学和EPR实验的数据可以结合起来并整体拟合到一个单一的动力学模型中。
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