Bisschop A, Vankan P M, Uijtewaal B, van Wijk R
Cancer Lett. 1984 Jun;23(2):151-7. doi: 10.1016/0304-3835(84)90148-4.
Intraperitoneal injection of the non-phorbol tumor promoter anthralin (1,8-dihydroxy-9-anthrone) in male rats resulted in an increase of hepatic ornithine decarboxylase (ODC) activity. Maximal activity was observed 8 h after promoter administration reaching levels about 30 times over control. The kinetics of anthralin dependent ODC induction differed markedly from that by either 12-O-tetradecanoylphorbol-13-acetate (TPA) or phenobarbital (PB) (Bisschop et al., Carcinogenesis 2 (1981) 1282). With anthralin a slow decrease of ODC back to control level is observed approximately within 22 h. In contrast, ODC induction mediated by other tumor promoters like TPA and PB decreased to control levels within 4-6 hours. Administration of a second dose of anthralin 8 h after the first dose prevented the activity decrease as normally observed after a single dose of a tumor promoter. This effect lasted at least 10 h. ODC activity induction occurred in a dose-dependent manner being linear from 10-2000 micrograms anthralin/kg body wt. Pretreatment of the animals either with actinomycin D or with cycloheximide completely blocked anthralin mediated ODC induction suggesting that de novo ODC-mRNA synthesis and subsequent translation is involved in this process.
给雄性大鼠腹腔注射非佛波醇肿瘤启动剂蒽林（1,8 - 二羟基 - 9 - 蒽酮）会导致肝脏鸟氨酸脱羧酶（ODC）活性增加。在给予启动剂后8小时观察到最大活性，达到比对照高约30倍的水平。蒽林依赖性ODC诱导的动力学与12 - O - 十四酰佛波醇 - 13 - 乙酸酯（TPA）或苯巴比妥（PB）明显不同（Bisschop等人，《癌变》2（1981）1282）。使用蒽林时，ODC会在约22小时内缓慢降至对照水平。相比之下，由其他肿瘤启动剂如TPA和PB介导的ODC诱导在4 - 6小时内降至对照水平。在第一剂蒽林给药8小时后给予第二剂蒽林可防止像单次给予肿瘤启动剂后通常观察到的活性下降。这种作用至少持续10小时。ODC活性诱导呈剂量依赖性，在10 - 2000微克蒽林/千克体重范围内呈线性关系。用放线菌素D或环己酰亚胺预处理动物会完全阻断蒽林介导的ODC诱导，这表明从头合成ODC - mRNA及其后续翻译参与了这一过程。
