Pereira M A, Chang L W, Ferguson J L, Couri D
Chem Biol Interact. 1984 Sep 1;51(1):115-24. doi: 10.1016/0009-2797(84)90024-3.
The products of the covalent binding of chloroform to rat hemoglobin during microsomal metabolism were isolated and identified by gas chromatography (GC) and mass spectroscopy (MS). After isolation by Proteinase K hydrolysis, amino acid analysis and cellulose thin-layer chromatography (TLC), the major product was identified by GC/MS as N-hydroxymethyl cysteine and a minor product as 2-hydroxythiazolidine-4-carboxylic acid. N-Hydroxymethyl cysteine is proposed to be formed during isolation from the 2-oxothiazolidine-4-carboxylic acid present in the intact hemoglobin.
在微粒体代谢过程中，氯仿与大鼠血红蛋白共价结合的产物通过气相色谱（GC）和质谱（MS）进行分离和鉴定。经蛋白酶K水解、氨基酸分析和纤维素薄层色谱（TLC）分离后，通过GC/MS鉴定出主要产物为N-羟甲基半胱氨酸，次要产物为2-羟基噻唑烷-4-羧酸。推测N-羟甲基半胱氨酸是在从完整血红蛋白中存在的2-氧代噻唑烷-4-羧酸分离过程中形成的。
