Khan-Dawood F S, Marut E L, Dawood M Y
Endocrinology. 1984 Aug;115(2):570-4. doi: 10.1210/endo-115-2-570.
To determine if oxytocin (OT) is present in cynomolgus monkey corpus luteum, OT was measured by a specific and sensitive RIA in 13 corpora lutea, ovarian venous plasma on the ipsilateral side and peripheral venous plasma at different stages of the luteal phase. Serial dilution of acetic acid extract of the corpus luteum showed parallelism with standard OT in the RIA. Total content of OT in corpus luteum was 1.9 +/- 0.5 ng (mean +/- SEM) with a content of 0.4-0.8 ng in early luteal phase, 1.0-6.2 ng in midluteal phase, and 0.4-0.7 ng in late luteal phase. OT concentrations in corpus luteum were 21.0-75.2 ng/g wet wt in early luteal phase, increasing to 34.4-602.5 ng/g in midluteal phase; and declining to 3.4-117.4 ng/g in late luteal phase. OT concentrations per mg protein in the corpus luteum were 0.05-19.6 ng with peak concentrations of 14.7-19.6 ng/mg protein on day 22. Sephadex G-25 column chromatography of the corpus luteum extract revealed a single peak for binding activity similar to that of synthetic OT on the RIA. Ovarian vein blood from the same side as the corpus luteum had a significantly higher OT concentrations of 161.2 +/- 29.7 pg/ml on days 15-24 than 16.8 +/- 3.6 pg/ml on days 25-28 (P less than 0.01) and peripheral plasma OT levels of 23.2 +/- 3.4 pg/ml (P less than 0.025). Our findings indicate that OT is present and probably produced by monkey corpus luteum with peak OT concentrations found in midluteal phase. Thus OT may play a role in primate corpus luteum function.
为了确定食蟹猴黄体中是否存在催产素（OT），采用特异性和灵敏的放射免疫分析法（RIA）对13个黄体、黄体期不同阶段的同侧卵巢静脉血浆和外周静脉血浆中的OT进行了测定。黄体醋酸提取物的系列稀释在RIA中显示与标准OT呈平行关系。黄体中OT的总含量为1.9±0.5 ng（均值±标准误），黄体早期含量为0.4 - 0.8 ng，黄体中期为1.0 - 6.2 ng，黄体晚期为0.4 - 0.7 ng。黄体中OT浓度在黄体早期为21.0 - 75.2 ng/g湿重，在黄体中期增至34.4 - 602.5 ng/g；在黄体晚期降至3.4 - 117.4 ng/g。黄体中每毫克蛋白质的OT浓度为0.05 - 19.6 ng，在第22天峰值浓度为14.7 - 19.6 ng/mg蛋白质。黄体提取物的葡聚糖凝胶G - 25柱层析显示，结合活性有一个单一峰，类似于RIA中合成OT的峰。与黄体同侧的卵巢静脉血在第15 - 24天的OT浓度显著高于第25 - 28天，分别为161.2±29.7 pg/ml和16.8±3.6 pg/ml（P＜0.01），外周血浆OT水平为23.2±3.4 pg/ml（P＜0.025）。我们的研究结果表明，OT存在于猴黄体中且可能由其产生，在黄体中期OT浓度达到峰值。因此，OT可能在灵长类动物黄体功能中发挥作用。
