Tseng L
Endocrinology. 1984 Aug;115(2):833-5. doi: 10.1210/endo-115-2-833.
Isolated human endometrial stromal cells were cultured in RPMI 1640 medium containing insulin, antibiotics and 10% fetal calf serum. After the stromal cells developed a confluent monolayer, estradiol (E2), progesterone (P), or both were separately added to the culture medium and cells were continuously cultured for various periods of time. Aromatase activity was measured in control and hormone-treated cells by incubating the intact cells with [3H]testosterone and isolating the estrogens at the end of incubation. E2 alone did not change the aromatase activity. P caused an 8- to greater than 40-fold increase over the control values (1 to 7 fmol/mg protein X h), and the activity was further increased in the presence of E2 (20- to 100-fold). The stimulation of aromatase activity by P was found to be both time- and dose-dependent and blocked by actinomycin D. Maximal stimulation was reached after the stromal cells were treated with 300 nM P for 3 days. At 30 nM P, a concentration similar to the plasma level during the luteal phase of the menstrual cycle, 80% of maximal stimulation was noted. These results indicate that P stimulates aromatase in endometrial stromal cells and E2 potentiates this stimulation. Much smaller effects of E2 and P on the aromatase activity were noted in endometrial epithelial glands.
将分离出的人子宫内膜基质细胞培养于含有胰岛素、抗生素和10%胎牛血清的RPMI 1640培养基中。当基质细胞形成汇合的单层细胞后，将雌二醇（E2）、孕酮（P）或两者分别添加到培养基中，并将细胞连续培养不同时间段。通过将完整细胞与[3H]睾酮孵育，并在孵育结束时分离雌激素，来测定对照细胞和激素处理细胞中的芳香化酶活性。单独使用E2不会改变芳香化酶活性。P使活性比对照值（1至7 fmol/mg蛋白×小时）增加了8倍至超过40倍，并且在存在E2的情况下活性进一步增加（20倍至100倍）。发现P对芳香化酶活性的刺激具有时间和剂量依赖性，并且被放线菌素D阻断。在用300 nM P处理基质细胞3天后达到最大刺激。在30 nM P（与月经周期黄体期的血浆水平相似的浓度）下，观察到最大刺激的80%。这些结果表明，P刺激子宫内膜基质细胞中的芳香化酶，而E2增强这种刺激。在子宫内膜上皮腺体中，观察到E2和P对芳香化酶活性的影响要小得多。
