Prokazova N V, Kocharov S L, Shaposhnikova G I, Zvezdina N D, Bergelson L D
Eur J Biochem. 1984 Jun 15;141(3):527-9. doi: 10.1111/j.1432-1033.1984.tb08224.x.
The glycolipid composition of Ehrlich ascites carcinoma cells was found to depend strongly on the cell density of the suspension. The general trend observed upon dilution of the cell suspension was a reduction of the less complex gangliosides GM3 and GM2 with concomitant increase of the more complex gangliosides, especially GM1. The increase of the content of ganglioside GM1 upon dilution was accompanied by a comparable decrease of the content of its immediate precursor, asialo-GM1, whereas the content of other neutral glycosphingolipids did not change very much. When the cell suspension was diluted with medium conditioned by dense cells the ganglioside profile of the diluted suspension remained similar to that of the dense cell suspension. It is postulated that the medium conditioned with dense cells contains a transferable factor inhibiting sialylation of asialo-GM1.
发现艾氏腹水癌细胞的糖脂组成在很大程度上取决于悬浮液的细胞密度。在稀释细胞悬浮液时观察到的总体趋势是，较简单的神经节苷脂GM3和GM2减少，同时较复杂的神经节苷脂，尤其是GM1增加。稀释后神经节苷脂GM1含量的增加伴随着其直接前体去唾液酸GM1含量的相应减少，而其他中性糖鞘脂的含量变化不大。当用高密度细胞条件培养液稀释细胞悬浮液时，稀释后悬浮液的神经节苷脂谱与高密度细胞悬浮液的相似。据推测，高密度细胞条件培养液中含有一种可转移因子，可抑制去唾液酸GM1的唾液酸化。
