Minami M, Togashi H, Sano M, Saito I, Nomura A, Koike Y, Kurosawa M, Saito H
Nihon Yakurigaku Zasshi. 1984 Apr;83(4):363-71.
A chronobiological study of behavioral changes in Wistar Kyoto rats (WKY), spontaneously hypertensive rats (SHR) and stroke prone spontaneously hypertensive rats (SHRSP) was undertaken. Attention was focused on changes in ambulatory activity and drinking behavior using an Ambulo-Drinkometer, the effects of centrally acting antihypertensive drugs, and the relationship between behavioral changes and humoral factors. (1) The experiment was performed during a light-dark alternation cycle. Approximately 10 days were required for rats to acclimate to new cages. (2) Using power spectral analysis, a 24 hr periodicity was dominant in both sexes of WKY and SHRSP. A 120 hr periodicity was demonstrated in female SHRSP. This long periodicity may have been due to a female sexual rhythm. (3) Ambulatory activity of WKY and SHRSP tended to decrease with age. (4) After abrupt cessation of clonidine administration, an ambulatory ultradian rhythm was demonstrated. Guanfacine treated SHR showed less change in ambulatory ultradian rhythm than clonidine treated SHR. (5) Using a Drinkometer with attached metabolic cages, drinking counts, urinary volumes, urinary aldosterone and catecholamine excretion rates were higher during the dark phase than during the light phase. It was demonstrated that analysis of rhythmicities and measurement of behavioral amplitude are both needed in the study of behavioral pharmacology.
对Wistar Kyoto大鼠（WKY）、自发性高血压大鼠（SHR）和易中风自发性高血压大鼠（SHRSP）的行为变化进行了一项时间生物学研究。研究重点是使用活动-饮水计观察活动量和饮水行为的变化、中枢性抗高血压药物的作用以及行为变化与体液因素之间的关系。（1）实验在明暗交替周期内进行。大鼠适应新笼子大约需要10天。（2）使用功率谱分析，WKY和SHRSP的雌雄两性均以24小时周期性为主。雌性SHRSP表现出120小时的周期性。这种长周期性可能归因于雌性的性节律。（3）WKY和SHRSP的活动量倾向于随年龄增长而减少。（4）可乐定给药突然停止后，出现了活动的超日节律。胍法辛治疗的SHR在活动超日节律方面的变化小于可乐定治疗的SHR。（5）使用带有代谢笼的饮水计，黑暗期的饮水次数、尿量、尿醛固酮和儿茶酚胺排泄率高于光照期。结果表明，在行为药理学研究中，节律分析和行为幅度测量都是必要的。
