McCracken A A, Emmett M, Crowle A J, Brown J L
Hepatology. 1984 Jul-Aug;4(4):715-21. doi: 10.1002/hep.1840040426.
We have used crossed immunoelectrophoresis to identify and establish the relative amounts of serum proteins secreted by a differentiated cell line (Fao) derived from a Reuber H35 rat hepatoma. Our results show that these cells secrete at least 15 plasma proteins. Ten of these: albumin, alpha 1-antitrypsin, alpha 1-lipoprotein, alpha 1-macroglobulin, alpha 1-antichymotrypsin, GC-globulin (transcalciferin), fibronectin, hemopexin, transferrin and the C3 component of complement have been identified. To examine the feasibility of using the Fao cell line as a model for studies on the regulation of hepatic protein secretion, we measured the relative amounts of 10 serum proteins secreted into the growth medium after exposure of these cells to dibutyryl cyclic AMP, hydrocortisone and a combination of both compounds. We also examined the effects of growth temperature (33.5 degrees, 37 degrees and 39 degrees C) and the removal of fetal calf serum from the growth medium on the relative amounts of these proteins secreted. We found that the rates of secretion of most of the serum proteins were altered by one or more of the treatments used in these experiments. In addition, detectable levels of secretion of three serum proteins, fibronectin and two unidentified, occurred only under certain of the experimental conditions. These results demonstrate that the pattern of proteins secreted from Fao cells can be experimentally altered and indicate that this cell line may be a useful model for studies on the control of hepatic protein secretion.
我们运用交叉免疫电泳技术来鉴定并确定源自鲁伯H35大鼠肝癌的分化细胞系（Fao）所分泌的血清蛋白的相对含量。我们的结果显示，这些细胞至少分泌15种血浆蛋白。其中已鉴定出10种：白蛋白、α1-抗胰蛋白酶、α1-脂蛋白、α1-巨球蛋白、α1-抗糜蛋白酶、GC球蛋白（转钙蛋白）、纤连蛋白、血红素结合蛋白、转铁蛋白以及补体C3成分。为检验将Fao细胞系用作研究肝脏蛋白分泌调节模型的可行性，我们测定了这些细胞在暴露于二丁酰环磷腺苷、氢化可的松以及这两种化合物的组合后分泌到生长培养基中的10种血清蛋白的相对含量。我们还研究了生长温度（33.5摄氏度、37摄氏度和39摄氏度）以及从生长培养基中去除胎牛血清对这些蛋白分泌相对含量的影响。我们发现，这些实验中使用的一种或多种处理改变了大多数血清蛋白的分泌速率。此外，仅在某些实验条件下才检测到三种血清蛋白（纤连蛋白和另外两种未鉴定的蛋白）的可检测分泌水平。这些结果表明，Fao细胞分泌的蛋白模式可通过实验改变，这表明该细胞系可能是研究肝脏蛋白分泌控制的有用模型。
