Perry R H, Candy J M, Perry E K, Thompson J, Oakley A E
J Anat. 1984 Jun;138 ( Pt 4)(Pt 4):713-32.
The substantia innominata was investigated in the adult human brain with particular reference to the cholinergic nature of the nucleus of Meynert. Based on histochemical observations of acetylcholinesterase activity and biochemical estimations of choline acetyltransferase the relations of the Meynert nucleus to adjacent structures in the substantia innominata region were identified precisely. A new dissection procedure is described which permits combined histochemical and histological examination of anatomically complex regions of the human brain, such as the substantia innominata, to be carried out in conjunction with accurate tissue dissection for neurochemical analysis. Using this technique, various acetylcholinesterase-positive and choline acetyltransferase-containing structures are apparent in coronal sections removed from the rostrocaudal length of the substantia innominata. These include, in addition to the prominently stained, putative cholinergic neurons, acetylcholinesterase-positive tracts which contain putative cholinergic projections to the neocortex, and 'islands' of acetylcholinesterase-positive neuropil which presumably reflect a collateral or intrinsic cholinergic innervation in the area. This anatomical complexity of cholinergic structures in the substantia innominata suggests that neurochemical analysis should be conducted on microdissected as opposed to macrodissected tissue samples. Neuropathologically, the present report provides a further basis for optimising quantification of putative cholinergic perikarya. Continued systematic analysis of the nucleus of Meynert at the morphological and biochemical level should thus establish the role of this nucleus in normal brain function and in disease.
对成人大脑的无名质进行了研究，特别关注了迈内特核的胆碱能性质。基于乙酰胆碱酯酶活性的组织化学观察和胆碱乙酰转移酶的生化测定，精确确定了迈内特核与无名质区域相邻结构的关系。描述了一种新的解剖程序，该程序允许对人脑中解剖结构复杂的区域（如无名质）进行组织化学和组织学联合检查，同时结合用于神经化学分析的精确组织解剖。使用该技术，在从无名质的前后长度切取的冠状切片中，可以看到各种乙酰胆碱酯酶阳性和含有胆碱乙酰转移酶的结构。除了染色明显的假定胆碱能神经元外，这些结构还包括含有假定向新皮质胆碱能投射的乙酰胆碱酯酶阳性束，以及乙酰胆碱酯酶阳性神经毡的“岛”，这些“岛”可能反映了该区域的侧支或内在胆碱能神经支配。无名质中胆碱能结构的这种解剖复杂性表明，神经化学分析应该对显微解剖而非大体解剖的组织样本进行。在神经病理学方面，本报告为优化假定胆碱能核周体的定量提供了进一步的依据。因此，在形态学和生化水平上对迈内特核进行持续的系统分析，应该能够确定该核在正常脑功能和疾病中的作用。
